Institute of Food and Radiation Biology

Plant Biotechnology and Genetic Engineering Division (PBGED)

Phone: 880-2-779 00 29, Fax: 880-2-7789620

Objectives

  1. To improve/multiply plants of economic importance using tissue culture and/ or mutation techniques.
  2. To establish and exploit genetic transformation techniques for the improvement of specific characters in plants of interest.

Activities

Projects

  1. Improvement of banana (Musa spp.) through in vitro mutagenesis and doubled haploids.
  2. Improvement of yield and protein content in salt tolerant rice landraces using induced mutation and/ or in vitro mutagenesis.
  3. Evaluation and improvement of Jatropha curcas for bio-diesel production.
  4. Establishment of micropropagation techniques in overexploited / endangered plant species.
  5. Establishment of genetic transformation techniques in sugarcane, mustard, rice, etc.

Achievements

  1. In vitro cloning systems of a number of forest, fruit, medicinal and ornamental/flowering plants have been developed -

    Forest trees: Banyan (Ficus benghalensis), Sisoo (Dalbergia sissoo), Teak (Tectona grandis), Peepul (Ficus religiosa), Ipil-ipil (Leucaena leucocephala), Koroi (Albizia procera) etc.

    Fruit plants: Carambola (Averrhoa carambola), Apple (Malus domestica), Kul (Zizyphus jujuba), Johnís Bread (Ceratonia siliqua), Wood apple (Aegle marmelos), Banana (Musa spp.), Orange (Citrus reticulata), Lemon (Citrus limon) etc.

    Medicinal plants: Neem (Azadirachta indica), Nishinda (Vitex negundo), Kurchi (Holarhena antidysenterica), Periwinkle (Catharanthus roseus), Kalokesi (Eclipta alba), Tulsi (Ocimum sanctum), Lajjabati (Mimosa pudica) Thankuni (Centella asiatica), Sheuli (Nyctanthes arbortristis), Dandakalosh (Leucas aspera), Khayer (Acacia catechu), Thuja (Thuja occidentalis) etc.

    Ornamental/flowering plants: Rose (Rosa spp.) and Orchids.

  2. Regeneration of plants from anthers of diploid banana cultivar has been reported for the first time in the country.
  3. Foreign genes have been successfully introduced into jute (Corchorus capsularis var. D-154) and Wood apple (Aegle marmelos) cells through Agrobacterium-mediated genetic transformation. This was confirmed by histochemical GUS-assay.
  4. Agrobacterium-mediated genetic transformation in tobacco (Nocotiana tabacum) and potato (Solanum tuberosum) has been established. Transgenic tobacco plants were successfully grown in potted soil.

Major Facilities

  1. Light and temperature controlled growth room of 200 sq. ft.
  2. Light and temperature controlled growth cabinets
  3. Laminar Flow Benches
  4. Autoclaves
  5. Ultra cold freezer (-800C)
  6. Low temperature incubator
  7. Fluorescent, phase-contrast and stereo microscopes with photographic attachments
  8. Gel electrophoresis units with power supply
  9. Shaking water bath

Perspective Plan

  1. Organogenesis and somatic embryogenesis in Papaya, Carica papaya L.
  2. In vitro micropropagation of Gerbera spp. from capitulum explant via callus.
  3. Efficient plantlet regeneration from bulb-scale of tuberose (Polianthes tuberosa L.) through in vitro culture and mutagenesis by gamma irradiation.
  4. In vitro micropropagation of Anthurium spp. from leaf explants via callus.
  5. Induction of mutants having the characteristics of variation in flower colour and extended vase life of different orchid cultivars.
  6. Improvement of oilseed Brassica spp. through genetic transformation.
  7. Study on organogenesis and Agrobacterium mediated transformation in Sugarcane, Sachharum officinarum L.
  8. Agrobacterium mediated genetic transformation in Papaya, Carica papaya L.
  9. Establishment of a green house at IFRB compound.
  10. Establishment of a bio-diesel plant for laboratory scale porduction of bio-diesel from jatropha seeds (oil).

Publications

National Indexed Journal

  1. S.N. Hossain, M.K. Munshi, M.R. Islam, L. Hakim and M. Hossain. 2003. In vitro propagation of Plum (Zyziphus jujuba Lam.). Plant Tissue Cult. 13(1): 81-84.
  2. S.N. Hossain, L. Hakim, M.R. Islam and M. Hossain. 2003. In vitro propagation of Karobi (Nerium indicum Mill.) using shoot tip of mature plant. Nucl. Sci. Appl. 12(1, 2): 107-111.
  3. M.K. Munshi., S.N. Hossain., M.R.Islam., G.Ahmed and L. Hakim. 2004. In vitro mass propagation of turmeric (Curcuma longa L.) using rhizome bud. Bangladesh J. Bot. 33(2): 125-128.
  4. S.N. Hossain, L. Hakim, M.R. Islam, G. Ahmed and M. Hossain. 2004. In vitro propagation of Leucas aspera (Willd) Link Ė an endangered medicinal plant. Nucl. Sci. Appl. 13 (1, 2): 103-107.
  5. P.K. Roy, A.N.K. Mamun and Golam Ahmed 2004. In vitro Plantlets Regeneration of Rose. Plant Tissue Culture. 14(2): 149-154.
  6. Hasina Akhter, L. Hakim, G. Ahmed, P.K. Roy and M.R. Islam. 2005. Study on regeneration and transformation efficiency of rice variety BR29. Nucl. Sci. Appl. 14(2): 96-99.
  7. S.N. Hossain, L. Hakim, G. Ahmed, M.R. Islam and M. Hossain. 2005. In vitro propagation of Coral Jasmine (Nyctanthes arbortristis L.) from nodal segments of mature plant. Nucl. Sci. Appl. 14(2): 92-95.
  8. S.N. Hossain, L. Hakim, G. Ahmed, M.R. Islam and M. Hossain. 2006. In vitro plant regeneration in Phyla nodiflora L. Nucl. Sci. Appl. 15(1): 76-79.
  9. S.N. Hossain, L. Hakim, G. Ahmed, M.R. Islam and M. Hossain. 2006. In vitro morphogenesis in Citrus limon L. Nucl. Sci. Appl. 15(1): 124-127.
  10. M.H. Kabir, P.K. Roy and Golam Ahmed. 2006. In vitro propagation of Thuja occidentalis through apical shoot culture. Plant Tissue Cult. & Biotech. 16: 5-9.
  11. A. N. K. Mamun. 2006. Reverse orientation of aldehyde dehydrogenase (ALDH) gene under TA29 promoter in tobacco plants Bangladesh J. Genet. Biotechnol 7(1&2): 43-48.
  12. A. N. K. Mamun. 2006. Antisene approaches of tobacco and potato ADP-glucose pyrophosphorylase (AGPase) under TA29 and NTM19 promoter in tobacco plants Bangladesh J. Genet. Biotechnol. 7(1&2): 57-60.
  13. M. K. Munshi, P.K. Roy, M.H. Kabir and G. Ahmed. 2007. In vitro Regeneration of Cabbage (Brassica oleracea L. var. Capitata) through Hypocotyl and Cotyledon Culture. Plant Tissue Cult. & Biotech. 17(2): 131-136

International Journal & Contribution to Book

  1. L. Hakim. 2003. Study of linkage relationships of Randomly Amplified Polymorphic DNA (RAPD) markers in a doubled haploid population of barley. Intl. J. Agric. Biol. 5(4): 526-529.
  2. M.K. Munshi, L. Hakim, M.R. Islam and Golam Ahmed. 2004. In vitro clonal propagation of banyan (Ficus benghalensis L.) through axillary bud culture. Intl. J. Agric. Biol. 6(2): 321-323.
  3. A.N.K. Mamun, M.N. Matin, M.A. Bari, N.A. Siddique, R.S. Sultana, M.H. Rahman and A.S.M. Musa (2004). Micropropagation of Woody Legume (Albizia lebbeck) Through Tissue Culture. Pakistan Journal of Biological Sciences. 7(7): 1099-1103.
  4. M.F.Alam, P. Sinha and M.L. Hakim. 2006. Mass clonal propagation of Vanda teres (Roxb) through in vitro culture of nodal segments. Propagation ornamental plants 6(3): 140-144.
  5. P. Sinha, M.L. Hakim and M.F. Alam. 2006. High frequency regeneration of Cymbidium through nodal segment-induced protocorms. In: Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Vol. 4, (First edition), J. A. Teixeira da Silva (ed.), Global Science Books Ltd, London, pp. 591-594.
  6. P. Sinha, M.L. Hakim and M.F. Alam. 2006. Continuous high frequency regeneration of different Phalaenopsis cultivars from young leaves of mature plants. In: Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Vol. 4, (First edition), J. A. Teixeira da Silva (ed.), Global Science Books Ltd, London, pp. 595-598.
  7. P. Sinha, M.L. Hakim and M.F. Alam. 2007. Efficient micropropagation of Phalaenopsis amabilis (L.) BL. cv. Cool Breeze using inflorescence axis thin sections as explant. Prop. Ornamental Plants 7(1): 09-15.
  8. P. Sinha, M.L. Hakim and M.F. Alam. 2007. Clonal propagation of white flowered hybrid Cattleya through in vitro culture of thin cell layers from young shoots of mature plant. Orchid Science and Biotechnology 1(1): 24-28.
  9. A. N. K. Mamun. 2007. Reversible male sterility in transgenic tobacco carrying a dominant-negative mutated glutamine synthetase gene under the control of microspore-specific promoter. Indian Journal of Experimental Biology 45: 1022-1030.
  10. Alexandra Ribarits, A. N. K. Mamun, Shipeng Li, Tatiana Resch, Martijn Fiers, Erwin Heberle-Bors, Chun-MingLiu and Alisher Touraev (2007) Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in dveloping anthers and pollen. Plant Biotechnology Journal 5: 483-494.

Other Information



Copyright © Atomic Energy Research Establishment, Ganakbari, Savar, P.O. Box No. 3787, Dhaka-1000, Bangladesh.
Telephone 880-2- 7789252; Facsimilie 880-2-7789337.