Citric Acid Production

(Some of the technologies may be available after suitable arrangements are made with AERE / BAEC authorities.)

	Citric Acid can be produced  by fermentation  of Aspergillus niger 


''Citric Acid Production Process by newly developed mutant strain GR318 of Aspergillus niger from cane sugar molasses medium'' have been patented.


It has a variety of uses. About 70% of citric acid produced is used in the food and beverage industry, about 12% pharmaceutical about 18% in other industrial applications.


Bangladesh at present annually imports more than 86,000 kg involving foreign currencies as per Bangladesh Bureau of Statistics. This requirement will be increased with the development of pharmaceuticals and other relevant industries.

With a view to producing citric acid locally by using cane molasses a waste product of sugar mill, an industrial potential mutant strain GR318 have been isolated by the treatment of gamma radiation from the parent strain CA16 of Aspergillus niger at the Institute of Food and Radiation Biology.

Potentiality of the mutant stain GR318

  1. This mutant strain could ferment raw material molasses, a waste product of sugar mill used for fermentation medium for citric acid production. It is readily available and is very cheap.
  2. Molasses usually contains many organic inhibitors and inorganic metallic ions for which the ability of Aspergillus niger to ferment sugar to citric acid declines substantially. This is usually removed by chemical treatment before fermentation. The developed mutant strain GR318 has good ability to ferment molasses to citric acid without any chemical treatment
  3. No need of pH adjustment (generally in molasses pH is 5.3 to 5.4 in which the mutant strain GR318 capable of producing more citric acid).
  4. At temperature 30c0 the strain GR318 is capable of producing 85% citric acid in surface culture condition. This 30c0 incubation temperature is effective for maximum period in Bangladesh. So, no need of any incubator for fermentation.
  5. The whole process could be carried out by utilizing simple equipment which will help in the economic production citric acid from cane molasses medium.
  6. This strain ferments in a shorter period (Peak day 9) gives 6.5 times more citric acid (85%) than the parent strain CA16(13%). which is suitable for commercial production in Bangladesh.

Contact us:
Director IFRB
Atomic Energy Research Establishment ( AERE )
Ganakbari, Savar

Tel , Director : 88-02-7701340
AERE Fax : 88-02-7701337, 88-02-7701620.

Or Contact;
Director General, AERE
Tel : 88-02-7701252, 88-02-7702165
Fax : 88-02-7701252
AERE Fax : 88-02-7701337, 88-02-7701620

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Telephone 880-2- 7701252; Facsimilie 880-2-7701337.