Tissue Banking & Biomaterial Research Unit

Tissue Banking & Biomaterial Research Unit

Phone: 88-02-,7789679 Fax: 88-02-7789034, 7789337

In Bangladesh, a large number of patients are suffering from some diseases and different accident like degenerative bone diseases, congenital deformities, fractures, non-union, bone loss from traumatic accidents, burns, acid violence etc. To rehabilitate these sorts of health problems, Tissue Banking and Biomaterial Research Unit (TBBRU) provided radiation sterilized amnion membrane and bone allografts routinely to the different hospitals/ clinics through out the country. By following scientific procedure, gamma irradiation (60Co) is used for tissue graft sterilization. This unit is also undertaken research activities on both the basic and applied/clinical areas of tissue banking.

Research and development work on Tissue Banking was initiated in late 1985 at the Institute of Food and Radiation Biology, AERE, Bangladesh Atomic Energy Commission under Co-ordinated Research Programme (CRP 4158/RB) of International Atomic Energy Agency (IAEA). A development project was implemented to strengthen Tissue Banking Laboratory during 1999-2004 at a cost of 4.0 crore taka under ADP of the Government of the People’s Republic of Bangladesh. Tissue Banking and Biomaterial Research Unit (TBBRU) was reformed as an independent unit under the Atomic Energy Research Establishment (AERE) in 2003. At present, the tissue banking research and development activities are being carried out as a regular research and development programme of Bangladesh Atomic Energy Commission.

Laboratory activities

Proposed Divisions

  1. Tissue allografts processing and quality control section
  2. Research & Development and coordination section
  3. Administration, Accounts and Maintenance section.

Aims and Objectives

  1. Processing of radiation (Gamma ray) sterilized human tissue allografts (e.g. amnion membrane, bone etc.) for use in rehabilitative surgery.
  2. Conducting research on radiation sterilization of tissue allografts and the impact of preservation, gamma irradiation on the biological, biomechanical and biochemical properties of tissue allografts.
  3. Conducting research on the processing of xenografts (origin from animal) and on developing biomaterials for clinical use.
  4. Culturing human cells and tissues for transplantation.
  5. Launching nationwide public and professional awareness programmes to motivate people for tissue donation and to disseminate information of tissue transplantation among the medical professionals.
  6. Establishing a National Tissue Bank/National Institute of Tissue Banking & Biomaterial Research.

Achievements

  1. Data showing the tissue allografts preparation, clinically utilization and patients treated during the period 1988-2007
    Types of tissue allograftsNo. of grafts processed No. of grafts used clinicallyNo. of patients treated
    Freeze/Oven dried Radiation sterilized amnion membrane30,00028,8002875
    Freeze dried Radiation sterilized bone allografts (chips/ block)18,00017,9001705
    Freeze dried Radiation sterilized demineralized bone granules700 vials675 vials220
    Cryo/deep Frozen-irradiated massive bone allografts361818
    TOTAL48,73645,6934,818

  2. At present more than 250 surgeons/physicians of 85 different hospitals/clinics/healthcare centers throughout the country are co-operating with research activities.
  3. National : Six seminars were organized in collaboration with different Medical College Hospital in different organizational environment.
    National : Four national Seminar was organized in co-operation with RCA and Ministry of Science and Information & Communication Technology, Government of the Peoples Republic of Bangladesh.
    National : Four discussion meetings were organized in order to promote the professional awareness. International : Organized SAARC Workshop on “Tissue Engineering and Banking” under SAARC – Japan Special Fund, 9-11 September, 2002, Dhaka, Bangladesh
    - One Radio Talk was given on “ Tissue Donation, Banking and Clinical Application in in Rehabilitative Surgery”.
    - A video documentary film –“ Tissue Banking for Rehabilitation of Disabled” has been prepared and broadcasted/transmitted in National Television (BTV).
    - A video documentary film –“ Tissue Banking : A Success History” has been prepared and broadcasted/transmitted in National Television (BTV) and private channel.

Services Provided


Type of tissue allograftsScope of clinical application
Radiation sterilized amnion allograftBurn & plastic surgery : Burn (superficial, mixed burn, electric burn, acid burn), leprotic ulcer, bedsore, donor site reconstruction, vaginoplasty & abdominal wall reconstruction, traumatic open wounds and other skin wounds.
Radiation sterilized /cryopreserved amnion allograftEye surgery: chemical burn, pterigium, corneal ulcer, cornea transplantation, limbal cell deficiency etc.
Freeze dried radiation sterilized bone allograftOrhtopaedic & spinal surgery : bone fracture, traumatic bone loss, gap non-union, cystic lesion of bone/tumor, THR, arthrodesis, TB spine, congenital deformity and other orthopaedic & spinal defects.
Demineralized bone matrix/granulesOral & Maxillofacial surgery : periodontal defects, preprosthetic surgery, tumor, traumatic facial defects, dentoalveolar defects etc.

Perspective plan

Treatment of orthopaedic patient
  1. Processing of human skin allolgrafts for the treatment of deep burns and other skin wounds patients.
  2. Processing of tendons, ligaments, fascia etc. for use in reconstructive surgery.
  3. Processing and preservation of xenografts (calf bone, goat & sheep skin) and biomaterials for clinical use.
  4. Establishing a human cell culture laboratory to produce in vitro human cells (keratinocytes, chondrocytes etc.) for transplantation.
  5. Continuing programmes on public and professional awareness of tissue donation and transplantation.
  6. Establishing a full-fledged National Tissue Bank.

Publications

  1. M.S. Islam and Rehana Begum. Tissue Banking : Radiation Sterilization of Tissue graft for use of rehabilitative surgery. (a) Microbiological and radiation sensitivity studies of bacterial isolates obtained from human amniotic membranes. Bangladesh J. Microbial. Vol. 4(1), 21-25, 1987.
  2. Rehana Begum and M.S. Islam.Tissue Banking : Radiation Sterilization of Tissue graft for use of rehabilitative surgery. lll. A highly radio-resistant bacteria isolate obtained from human amniotic membrane, Bangladesh. J Microbial. Vol. 6 (2), 33-36, 1989.
  3. M.S. Islam, A. Nessa, R, Begum, M.H. Jamely and Dr. A. R. Khan- Tissue banking: Utilization of oven dried radiation sterilized amnion membrane grafts for treatment of burn patient in Bangladesh. A preliminary report. Dhaka Shishu (children) Hospital Journal. Vol. 3 No. 1 and2, June and December 1989.
  4. Rehana Begum, M.S. Islam and M. Shahidullah -Radiation Sterilization of Tissue graft for use in rehabilitative surgery. ll. Use of sterilized amnion membrane graft as temporary biological dressing for burn patients. Bangladesh Journal of Child Health.Vol. 4 (1),18-20,1990.
  5. M.S. Islam and Rehana Begum. Tissue Banking: Radiation Sterilization of Tissue graft for use of rehabilitative surgery. IV. Microbiological and radiation sensitivity studies of bacterial isolates obtained from cow bones. Bangladesh J. Microbial. Vol. 7 (2), 81-84, 1990.
  6. Dr. A. R. Khan, Dr. M. S. Islam, A. Nessa and R. Begum- Use of amniotic membrane dressing for burns in children: A cheaper and effective way of treatment. Bangladesh Private Medical practitioners journal. Vol 3, No. 1 1992.
  7. Dr. M. M. Bari, Rehana Begum, S. K. Chakraborty and M. S. Islam- Use of radiation sterilized Amniotic membrane grafts as temporary biological dressing for the treatment of leprotic ulcers. Acte Orthopaedica Hellencia, Vol. 46, No.1, Athens, Greece, 1995.
  8. Rehana Begum and M.S. Islam- Tissue Banking : Load of bacteria in human femur bones and their sensitivity to radiation. Nuclear Science and Application, Vol 5, No. 1 &2, 1996.
  9. G.A.S. Shamim, A.F.M. Ruhul Haque, M.A. Mannan and A. Nessa- Use of allograft in treating bone defect. Journal of Orthopaedic Society. Vol. 11, No. 1, 1996.
  10. F. Haque, A. Nessa, and M.S. Islam – Treatment of pressure sore with radiation sterilized amniotic membrane grafts. Dhaka Shishu (children) Hospital Journal, Vol. 15 No. 1 and2, June 1999.
  11. Rehana Begum–Tissue Banking: Radiation Sterilized tissue allografts (Human) in rehabilitative surgery. The Bangladesh Journal of Science and Technology. Vol. 2, No. 2, June 1999.
  12. Rehana Begum and A. Nessa- Preparation of human tissue grafts. Advances in Tissue Banking, Vol. 3, World Scientific, Singapore, pp 453-455, 1999.
  13. M. S. Islam and R. Begum- Microbiological and radiation sensitivity studies of bacterial isolates obtained from human amnion membranes. Advances in Tissue Banking, Vol. 3, World Scientific, Singapore, pp 457-462, 1999.
  14. A highly radio resistant bacterial isolate from amniotic membranes. Advances in Tissue Banking, Vol. 3, World Scientific, Singapore, pp 463-467, 1999.
  15. M. M. Bari and R. Begum-Use of radiation Sterilized Amniotic membrane grafts as temporary biological dressing for the treatment of leprotic ulcers. Advances in Tissue Banking, Vol. 3, World Scientific, Singapore, pp 477-483,1999.
  16. F. Jahan, R. Karim and R. Begum – Use of radiation sterilized freeze dried and deminerilized human bone grafts in dental surgery. Advances in Tissue Banking, Vol. 3, World Scientific, Singapore, pp 485-490,1999.
  17. Bari M.S., Choudhury M.I.M., Khan A.R., Nessa A.: Role of Human Foetal Membranes (Amniotic Membrane) in the Management of Burn Wounds. Annals of Burns and Fire Disasters Journal, Vol. XV, No. 4, Mediterranean burns Council, 2002.
  18. AR Khan, MA Aziz, MS Ullah, A. Nessa, SK Chakraborty, MM Miah: Radiation Sterilized amnion membrane grafts in the management of superficial burn in children. Dhaka Shishu (children) Hospital Journal, ISSN 1013-2295, Vol. 18 No. 2, pp 52-54, 2002.
  19. SK Chakraborty, A. Nessa, MM Miah, AR Khan, MA Aziz, M Saif ullah: Management of mixed (superficial and deep) burn with allogenic amnion and autogenic skin graft. Bangladesh J. Med. Sci., Vol 9, No 2, Sep 2003.
  20. SK Chakraborty, A. Nessa, and AKM Eshaque: Immunogenicity of allogenic bone grafting and minimizing immunogenic rejection of transplant bone allograft by improved processing technique. Bangladesh J. Med. Sci., Vol 10, No 2, Sep 2004.
  21. Nakul K Datta, Serajuddin Ahmed, Azizun Nessa and M. Mamun Miah: Application of lyophilized radiation sterilized bone allograft with autogenous bone marrow in treatment of cyst and cysts like lesions of bone . BJMC, ISSN # 1607-0755. Vol. 11, No. 01, March 05.
  22. Datta N. K., Nessa A. and Miah M.M.: Biological incorporation of freeze dried radiation sterilized bone allografts- study of 12 cases of Aneurosymal Bone Cyst lesions, Journal of Doctors Association of Bangladesh. ISSN # 1813-4726, Vol 02, pp39-43, 2005.
  23. SK Chakraborty, MZ Hasan, K Chakma, H Rashid, A Nessa and DJ Gomes: Inactivation of bacterial vegetative cells and endospores with some chemical disinfectants.Dhaka Univ. J. Biol. Sci., 16 (2): 109-115, 2007 July.


Copyright © Atomic Energy Research Establishment, Ganakbari, Savar, P.O. Box No. 3787, Dhaka-1000, Bangladesh.
Telephone 880-2- 7789252; Facsimilie 880-2-7789337.